Michel Gohar

- contact me:

E.mail: Michel Gohar

Téléphone: +33 (0)1 34 65 23 81

A. Education

Institution  Degree Year Field
Université de Poitiers, Poitiers, France PhD 1984 Biochemistry

Université de Poitiers, Poitiers, France Postdoc 1984-1985 Cellular Biology
Université de Paris XI, Orsay, France HDR 2009 Molecular Biology

B. Positions and Employment

- 1986-1991: Celaster SA, Celle l'Evescault, France. Subcontractant for pharmaceutical industries, Research Scientist

- 1991-2003: Aventis CropScience, Marseille, France. Crop protection Industry, Research Scientist

- 2003-present: INRA, Micalis UMR1319, Jouy-en-Josas, France. Government Research Institute, Research Scientist

C. Research Interests

Biofilm formation in B. cereus:

• Role in pathogenesis

• Regulation

• Cell-surface proteins and polysaccharides

• Multispecies biofilms

D. Reviewer activities for international projects

• 2007 - US-Israel Binational Agricultural Research and Development Fund

• 2009 - Canadian Forest Service Regulatory System For Biotechnology

• 2012 - US-Israel Binational Agricultural Research and Development Fund

E. Patents

• Demassey J., Gohar M., Wehrey C., 1995 - New aromatic amides, their preparation process, the compositions containing them and their use as pesticide. Patent n° WO1996022965 A1

• Briandet R., Houry A., Deschamps J., Gohar M., Gruss A., Aymerich S., 2012 - Kinetic energy of self-propelling bacteria as a biological tool to control hazard biofilms. Patent n° WO2012084746 A1

F. Current Research Support

• ANR grant, Bt-Surf, ANR-09-Blan-0253 03/01/2013 - 02/28/2015 Lereclus, D. (PI) Production and uses of Bacillus thuringiensis surfactants €75,000 (year)/ €135,000 (total/year).

• ANR grant, CRYMUC, APR RISK OGM 2010 02/01/2012 - 02/01/2015 Nielsen-Leroux, C. (PI) Interactions between insecticidal Cry toxins and digestive tract mucus €100,000 (year)/ €300,000 (total/year).

G. Peer-reviewed publications for the last 5 years (in reverse chronological order)

• Fagerlund A., Dubois T., Okstad O-A., Gilois N., Bennaceur I., Perchat S., Gominet M., Aymerich S., Kolsto A-B., Lereclus D., Gohar M. – SinR controls both enterotoxin and biofilm productions in Bacillus cereus, PLoS One. 9(1):e87532. doi: 10.1371/journal.pone.0087532.

• Kamar R., Gohar M., Jéhanno I., Réjasse A., Kallassy M., Lereclus M., Sanchis V., Ramarao N., 2013 – Pathogenic Potential of Bacillus cereus Strains as Revealed by Phenotypic Analysis, J. Clin. Microbiol., 51:320-323.

• Dubois T., Faegri K., Perchat S., Lemy C., Buisson C., Nielsen-LeRoux C., Gohar M., Jacques P., RamaRao N., Kolsto A-B, Lereclus D., 2012 – Necrotrophism Is a Quorum-Sensing-Regulated Lifestyle in Bacillus thuringiensis, PLoS Pathogens, 8:e1002629.

• Houry A., Gohar M, Deschamp J., Tischenko E., Aymerich S., Gruss A, Briandet R, 2012 – Bacterial swimmers that infiltrate and take over the biofilm matrix. Proc. Natl. Acad. Sc. USA, 109:13088-13093.

• Perchat S., Dubois D., Zouhir S., Gominet M., Poncet S., Lemy C., Aumont-Nicaise M., Deutscher J., Nessler S., Gohar M., Lereclus, D., 2011 – A cell-cell communication system regulates protease production during sporulation in bacteria of the Bacillus cereus group. Mol. Microbiol., 82:619-633.

• Candela T., Maes E., Garénaux E., Rombouts Y., Krzewinski F., Gohar M., Guerardel Y., 2011 – Environmental and biofilm-dependent changes in a Bacillus cereus secondary cell wall polysaccharide. J. Biol. Chem., 286:31250-31262.

• Tran S.-L., Guillemet E., Clybouw C., Morris A., Gohar M., Lereclus D., Rama Rao N., 2011 – Hemolysin II is a Bacillus cereus virulence factor that induces apoptosis of macrophages. Cell. Microbiol., 13 (1):92-108.

• Tran S.-L., Guillemet E., Gohar M., Lereclus D., Rama Rao N., 2010 – CwpFM (EntFM) is a Bacillus cereus potential cell wall peptidase implicated in adhesion, biofilm formation, and virulence. J. Bacteriol., 192:2638-2642.

• Guimaraes V., Drumare M.-F., Lereclus D., Gohar M., Lamourette P., Nevers M.-C., , Vaisanen-Tunkelrott M.-L., Bernard H., Guillon B., Créminon C., Wal J.-M., Adel-Patient K., 2010 – In vitro digestion of Cry1Ab proteins and analysis of the impact on their immunoreactivity. J. Agric. Food Chem., 58:3222-3231.

• Houry A., Briandet R., Aymerich S., Gohar M., 2010 – Motility and flagellum involvement in Bacillus cereus biofilm formation. Microbiology, 156(4):1009-1018.

• Daou N., Buisson C., Gohar M., Vidic J., Bierne H., Kallassy M., Lereclus D., Nielsen- LeRoux C., 2009 – IlsA, a unique surface protein of Bacillus cereus required for iron acquisition from heme, hemoglobin and ferritin. PLoS Pathogens, 5:e1000675.

• Auger S., RamaRao N, Faille C., Fouet A., Aymerich S., Lereclus D., Gohar M., 2009 – Biofilm formation and cell-surface properties in Bacillus cereus. Appl. Environ. Microbiol., 75(20):6616-6618.

• Brillard J., Susanna K., Michaud C. Dargaignaratz C., Gohar M., Nielsen-Leroux C., RamaRao N., Kolsto A.B., Nguyen-the C., Lereclus D., Broussolle V., 2008 – The YvfTU two-component system is involved in plcR expression in Bacillus cereus. BMC Microbiol., 8:183.

• Gohar M., Faegri K., Perchat S., Ragnum S., Økstad O.E., Gominet M., Gilois N., Kolstø A.B., Lereclus D., 2008 – The PlcR Virulence Regulon of Bacillus cereus, PLoS One, 3:e2793.

• Bouillaut L., Perchat S., Arold S., Zorrilla S., Slamti L., Henry C., Gohar M., Declerck N., Lereclus D., 2008 – Molecular basis for the specific TPR-mediated activation of PlcR with the heptapeptide PapR. Nucleic Acid Research, 36:3791-3801.